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RNPs are sent to CD34+ cells by way of the CellPore™ Transfection System. Article-transfection, cells are returned to tradition and subsequently analyzed for enhancing efficiency through recognized techniques as permitted because of the experimental style, for instance movement cytometry or ArciTect™ T7 Endonuclease I Package (Catalog #76022). Edited progenitor cell function could be calculated by in vitro colony-forming unit (CFU) assays or cells is often additional cultured for lineage-precise differentiation utilizing customized StemSpan™ media and nutritional supplements.
Move cytometry can be utilized to measure the performance of differentiation protocols While using the STEMdiff™ system, by thinking about certain marker expression at Just about every stage of the differentiation course of action. It can be used for other purposes, like cell sorting, immunophenotyping, and purity evaluation.
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This product is created for use in the following investigate location(s) as part of the highlighted workflow stage(s). Check out these workflows to learn more with regards to the other items we provide to assistance Each individual investigate spot. Investigate Location Workflow Levels
The readings can be performed prior to the tradition is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
There are many commercially out there resources for Main airway epithelial cells. We have examined HAECs from two suppliers, Lonza and Epithelix — the two have resulted in effective ALI cultures.
Note: Move forward to antibody staining if undertaking Reside cell Investigation after this phase. Alternatively, commence to fixable dye staining and PFA fixation.
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Commonly, HSAECs cultured in PneumaCult™-ALI-S Medium will Stem cell form a totally differentiated cuboidal epithelium immediately after four to five weeks of society. Some donor variability could possibly be anticipated.
Use 줄기세포 지방이식 cell counts to independent the sample into two tubes – “unstained” and “with viability dye”.
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